The method SPrOS (Specificity Projection On Sequence) is developed to analyze the amino acid sequences related to the same protein family in order to recognize the amino acid residues associated with separated subclasses within this family.
The algorithm SPrOS requires the training set of preliminary classified amino acid sequences. A user should choose the test sequence(s), in which class-specific positions to be predicted. The test sequence is excluded from the training set and compared with all the rest sequences. The obtained positional scores are used as input to the procedure, which estimates the specificity of each query sequence position to each given class
The method provides the specificity estimates Eia, evaluating the specificity of the position i of tested sequence to the class A. The more the Eia value the more the specificity evaluation. The p-values are used to obtain the statistical significance of the Eia estimates. The lower the p-value the more significant Eia.result.txt for example